Augmentation Award for Monoclonal Antibody Detection of Chlorinated Benzenes on Contaminated Sediments.
1997
O'Bannon, Deborah J.
The nonextractive immunoassay techniques developed with enzyme immunoassay procedures were extended to include fluorescent immunoassay of sorbed contaminants. The variety of substrates to which the contaminant was sorbed was increased to include St. Peters sandstone, montmorillonite, and black shale. Autofluorescence of the substrates was addressed, and 2,4-dinitrobenzene was found not to migrate into the montmorillonite crystal layers. Additional sample preparation was needed for the clay samples: significantly more blocking solution was needed to reduce background nonspecific binding of the immunochemicals. Two contaminants were used throughout the grant period -- 2,4,-dinitrobenzene, and pyrene.
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