Cloning and characterization of G protein beta 1 subunit in shrimp Litopenaeus vannamei | 凡纳滨对虾G蛋白Gβ_1亚基的克隆、表达与鉴定
2006
金利华 | 骆晶晶 | 林圣彩 | 叶志云
根据G蛋白Gβ1亚基的保守序列设计简并引物,通过简并PCR和RACE技术,克隆到凡纳滨对虾(Litopenaeusvannamei)Gβ1基因的全长cDNA序列。利用Blast、DNAstar和Genedoc软件分析,发现该Gβ1编码的蛋白序列与其他物种已知的Gβ1序列具有相当高的保守性,将它命名为pvGβ1。免疫共沉淀分析发现pvGβ1能在体外适当条件下与对虾Gαs或Gαq相互结合。Westernblot-ting分析发现,pvGβ1在对虾身体各部位都有广泛分布,尤其在脑神经、眼和眼柄有大量表达。说明了Gβ1在对虾的神经系统和光信号传导等生命过程中的重要性。 【英文摘要】 The β/γ-subunits of the heterotrimeric GTP-binding proteins are important regulators of G-protein alpha subunits as well as a series of signal transduction receptors and effectors. In this study, a novel G protein β_1 subunit was isolated from shrimp Litopenaeus vannamei, and was termed pvGβ_1. Sequence analysis showed that the pvGβ_1 protein contained all the well-conserved domains and motifs that were critical sites for interaction with receptors and other binding effectors. Co-immunoprecipitation assay d...
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