ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY BETWEEN MICROPROPAGATED AND FIELD GROWN PLANTS OF EXCOECARIA AGALLOCHA L.
2012
Manickam Arumugam | Umesh Ramachandra Pawar | Muthaiah Gomathinayagam | Ganapathy Murugan Alagu Lakshmanan | Rajaram Panneerselvam
Excoecaria agallocha has long been used in folk medicine in many countries as antimicrobials and/or antioxidants. Eighty percent methanol extracts obtained from Excoecaria agallocha was evaluated for antibacterial activity using the disc diffusion assay, antiradical scavenging effects, reduced glutathione and phenolic. Best antibacterial activity as indicated by disc diffusion zone values was obtained by micropropagated Excoecaria agallocha against Staphylococcus aureus with a disc diffusion zone 25mm. Micropropagated and field grown plants inhibited growth of Klebsiella pneumoniae with zone values of 13mm and 11mm in diameter respectively. A remarkable DPPH scavenging effect was observed with micropropagated plants (IC50 of 10.2, 10.8 and 17.4 μg/ml, respectively). Ascorbic acid (AA) content observed in micropropagated plant leaf 18 mgl-1plant-1 when compared to field grown plant ascorbic acid content. Highest total phenolic contents were recorded in micropropagated plants leave (207 and 205 mg/GAE/g). Our findings support the uses of the Excoecaria agallocha in traditional medicine. The interesting biological activities obtained by micropropagated plants of Excoecaria agallocha need to be further investigated to isolate active agents and to study their mechanism of action.
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