Biosynthesis of chitinolytinzimes by Paecilomyces marquandii and Paecilomyces lilacinus F-2 | Биосинтез хитинолитических ферментов Paecilomyces marquandii и Paecilomyces lilacinus F-2
2012
Tsyrkunova, Zh.F., [email protected] | Mihajlova, R.V., National Academy of Sciences (Belarus). Institute of Microbiology | Lobanok, A.G., National Academy of Sciences (Belarus). Institute of Microbiology | Khomich, M.B., National Academy of Sciences (Belarus). Institute of Microbiology
Effect of nutrient medium components on synthesis of chitinolytic enzymes by Paecilomyces marquandii and Paecilomyces lilacinus F-2 was investigated in the conditions of the Republic of Belarus. In course of the study there were presented results of formation of chitinolytinzimes by Paecilomyces marquandii and Paecilomyces lilacinus F-2 on the media with different chitine containing substrates. There was analysed influence of different carbonium sources on synthesis of chitinolytinzimes. Inducible type of chitinase and chitosanase production was demonstrated. It was foundthat supply of manganese, iron, zinc and cobalt salts into the medium activate denzyme synthesis in P. marquandii andP. lilacinus F-2. High level of chitinase and chitosanase generation in P. marquandii and chitinase production in P. lilacinus F-2 was recorder upon supplementation of the media with corn steep liquor and malt extract. Possibility of introducing fungal biomass, beet cake and wheat bran into the nutrient media for fermentation of strain-producers of chitinolytic enzymes was evaluated. It was established that production of chitinases by P. marquandii andP. lilacinus F-2 reached maximum by 5 days of culture, chitosanase synthesis – by 6 days.
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