Application of apple gene sequence for pear primer design | 苹果基因序列在砂梨引物开发中的应用
2011
Shi Zebin, Zhejiang Academy of Agricultural Sciences,Hangzhou(China),Institute of Horticulture | Wang Yuezhi, Zhejiang Academy of Agricultural Sciences,Hangzhou(China),Institute of Horticulture | Dai Meisong, Zhejiang Academy of Agricultural Sciences,Hangzhou(China),Institute of Horticulture
中国人. 摘 要：选取苹果液泡质子泵焦磷酸水解酶基因、液泡质子泵三磷酸腺苷酶A亚基基因、生长素受体基因、脱落酸受体基因、乙烯受体基因、果重基因、抗病相关基因等共8个基因,根据其基因组序列分别在起始密码子上游区、终止密码子下游区、外显子区和内含子区设计引物,用这些引物同时对苹果和砂梨的基因组DNA进行PCR扩增。结果显示：除了抗病相关基因,其余7个基因均有引物在砂梨基因组DNA中扩增出特异条带。与基因其他区域扩增产物相比,起始密码子上游区扩增产物在不同砂梨材料间多态性较丰富,外显子和内含子区扩增产物在不同砂梨材料间的保守性更高。
显示更多 [+] 显示较少 [-]英语. Apple vacuolar proton pump PPase,subunit A of ATPase,auxin receptor gene,ABA receptor gene,ethylene receptor gene and the genes involved in fruit weight regulation and disease resistance were selected for PCR analysis of apple（Malus domestica Borkh.）and pear（Pyrus pyrifolia）.For each gene,the primers were designed in exon and intron as well as in the regions upstream of start codon and downstream of stop codon,respectively.These primers were used to amplify apple and sand pear genomic DNA.It was found that all the genes have at least one pair of primers amplifying specific bands in pear genome except the disease-resistant gene.Compared to the products amplified at gene′s other regions,the PCR products at upstream of start codon showed more polymorphics,and the PCR products at exon and intron region showed higher conservation,among different sand pear genotypes.
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