Effects of clenbuterol hydrochloride on the structure of lung and antioxidant capacity of mice | 盐酸克伦特罗对小鼠肺脏组织结构及抗氧化能力的影响

英语. The effects of clenbuterol hydrochloride (CLH) on the lung were studied through the observation of the structure of lung and determination of anti-oxidative index. Mice were continuously administered with CLH at the doses of 12.5, 25.0 and 50.0 mg/kg and the same amount of saline for 30 days. The changes of the lung were observed by light microscope. Expression of Bax protein was measured by immunohistochemical method. At the same time, activities of SOD, CAT and content of MDA were detected on the 5th, 10th, 20th, 30th days. Compared with the control group, the level of SOD and CAT tended to reduce, while the level of MDA seemed to be elevated. The diameter of alveolous, thickness of interalveolar septum and surface area density of the positive expression of Bax protein significantly increased than that of the control. The degree of pathological changes was in dose and time-dependent effect. Results of Two-Way ANOVA indicated that dose contributed more to CLH-induced lung damage than treatment time. CLH could destroy the balance between oxidative stress and anti-oxidative function of the body, and this effect might be correlated with the low activities of SOD, CAT and high content of MDA in mice’s lung.

中国人. 通过对肺脏组织结构的观察及其抗氧化指标的测定，研究盐酸克伦特罗（CLH）对肺脏损伤的影响。给小鼠分别灌胃12.5，25.0，50.0 mg/kg的CLH连续30 d，对照组灌胃等量生理盐水，在灌胃处理5，10，20，30 d时用生物显微技术观察小鼠肺脏结构的变化，用免疫组织化学方法观察Bax蛋白在肺脏的表达，用比色法检测肺脏SOD、CAT活性及MDA含量的变化。与对照组比较，灌胃CLH小鼠肺脏SOD、CAT活性降低，MDA含量升高，肺泡直径、肺泡隔厚度、Bax蛋白阳性表达的面密度值升高；CLH对小鼠肺脏的影响具有时间-剂量效应；双因素方差分析表明，剂量因素对结果的影响大于时间因素。CLH可破坏机体氧化与抗氧化的动态平衡，对小鼠肺脏组织结构的损伤可能与肺脏中SOD、CAT活性的降低及MDA含量的升高有一定相关性。