Acute toxicity effects of m-dihydroxybenzene and o-dihydroxy benzene on Misgurnus anguillicadatus | 间苯二酚与邻苯二酚对泥鳅的急性毒性效应

中国人. 探讨间苯二酚(m-dihydroxybenzene)和邻苯二酚(o-dihydroxybenzene)对泥鳅(Misgurnus anguillicadatus)的毒性效应，为评价酚类化合物对水生生物的毒害作用提供理论依据。采用单因子急性毒性试验，分别检测不同质量浓度间苯二酚（10.00，12.10，14.50，17.40，20.90，25.20和30.20 mg/L）和邻苯二酚（20.00，28.05，39.45，55.46，77.98，109.60和154.20 mg/L）对泥鳅的急性毒性效应，观察泥鳅的中毒症状，统计死亡率，建立死亡几率与药物质量浓度常用对数值的回归方程，计算 2 种化合物对泥鳅的半致死浓度 (LC50)和安全浓度(SC)。泥鳅在高质量浓度(25.20，30.20 mg/L)间苯二酚中8 h 开始出现死亡，在高质量浓度邻苯二酚(109.60， 154.20 mg/L)中16 h 开始出现死亡；2 种化合物对泥鳅的毒性作用随其在体内积累质量浓度的增大、作用时间的延长而增强；间苯二酚对泥鳅 24，48，72和96 h的LC50分别为 27.04，23.44，20.91和17.40 mg/L，SC为5.29 mg/L；邻苯二酚对泥鳅24，48，72和96 h的LC50分别为 127.50，96.74，75.53和50.53 mg/L，SC 为 16.71 mg/L。间苯二酚与邻苯二酚均为高毒性污染物，且前者比后者的毒性更大；泥鳅群体对间苯二酚反应的同质性较高；生产中对这 2 种化合物的毒性应引起高度重视。

英语. This paper studied the acute toxicity of m-dihydroxybenzene and o-dihydroxybenzene to Misgurnus anguillicadatus, which can offer reference to evaluate acute toxicity of phenolic compound to hydrobiont. We used the methods of single factor toxicity test to detect the acute toxicity effects of m-dihydroxybenzene (10.00, 12.10, 14.50, 17.40, 20.90, 25.20, 30.20 mg/L) and o-dihydroxybenzene (20.00, 28.05, 39.45, 55.46, 77.98, 109.60, 154.20 mg/L) on Misgurnus anguillicadatus, observed their toxic symptoms, counted the death ratios, educed the regression equations and computed the median lethal concentrations (LD50) and the safety concentrations (SC) under seven concentration grads. The loaches begin to die after 8 hours in the m-dihydroxybenzene with high concentration (25.20, 30.20 mg/L) and after 16 hours in the o-dihydroxybenzene with high concentration (109.60, 154.20 mg/L). With the increasing concentration and exposure time of two compounds, their toxicity effects to M. anguillicadatus increased. The LC50 of m-dihydroxybenzene for 24, 48, 72, and 96 hours were 27.04, 23.44, 20.91 and 17.40 mg/L, respectively, and the SC was 5.29 mg/L; while the mean LC50 values of o-dihydroxybenzene for 24, 48, 72, and 96 hours were 127.50, 96.74, 75.53 and 50.53 mg/L, respectively, and the SC was 16.71 mg/L. Both m-dihydroxybenzene and o-dihydroxybenzene had high toxicity to M. anguillicadatus, and m-dihydroxybenzene had higher toxicity than o-dihydroxybenzene. The groups of M. anguillicadatus have a higher homogeneity reacting to m-dihydroxybenzene than o-dihydroxybenzene. The toxicity of two compounds should be paid to much attention.