Association of Polymorphism C1149G of Nuclear Enriched Abundant Transcript1 Gene(NEAT1) with Hematological and Immune Traits in Landrace Pig(Sus scrofa) | 长白猪NEAT1基因C1149G多态性位点与血液和免疫性状的关联
2012
Zhang Ningbo, Linyi University，Linyi(China)，College of Life Science | Li Yong, Chinese Academy of Agricultural Sciences，Beijing(China)，Institute of Animal Science | Jing Wenqian, Chinese Academy of Agricultural Sciences，Beijing(China)，Institute of Animal Science
中国人. NEAT1 是一个长的非编码RNA，参与了免疫反应、paraspeckle形成以及mRNA的输出等生物学过程。本研究利用PCR-RFLP方法检测了猪(Sus scrofa)NEAT1一处C1149G单核苷酸位点的多态性，在检测的猪品种中等位基因频率变异较大。关联分析结果表明，在长白猪群体中CC基因型个体，0日龄和17日龄猪瘟病毒(CSFV)抗体水平，0日龄平均红细胞体积和32日龄红细胞平均血红蛋白浓度显著高于GG或CG基因型；而32日龄的血小板分布宽度、血小板平均容积和大血小板比率则显著低于GG或CG基因型。实验结果可以为猪的分子标记和辅助育种提供参考。
显示更多 [+] 显示较少 [-]英语. Nuclear enriched abundant transcript 1(NEAT1) is a long non-coding RNA that is involved in immune response, paraspeckle formation and mRNA export. In this study, a single nucleotide polymorphism C1149G in porcine(Sus scrofa) NEAT1 was detected by PCR-RFLP. Allele frequencies varied greatly among different pig breeds examined, and the association results indicated that piglets with the genotype CC had significantly higher levels of Classical swine fever virus (CSFV) antibody (0 and 17 days), mean corpuscular volume(0 days) and mean corpuscular hemoglobin content (32 days), but lower platelet distribution width (32 days), mean platelet volume (32 days) and platelet larger cell ratio (32 days) than those with genotype GG or CG in a Landrace population. The results can be provided as references to molecular markers assisted breeding for pigs.
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