A simple method for isolating chloroplast dna and mitochondria dna from the same rapeseed green leaf tissue
2013
Hu Zhiyong, Chinese Academy of Agriculture Sciences, Wuhan (China), Oil Crops Research Institute | Zhan Gaomiao, Chinese Academy of Agriculture Sciences, Wuhan (China), Oil Crops Research Institute | Wang Hanzhong, Chinese Academy of Agriculture Sciences, Wuhan (China), Oil Crops Research Institute
In the study, we present a fast, simple and inexpensive protocol for isolating chloroplast and mitochondrial DNA from one rapeseed leaf tissue sample. The chloroplast and mitochondria were separated from the same green leaf tissue by differential centrifugations. The protocol is the first report that isolates plant chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) from the same sample homogenate. The organelle DNA yield is 2-10 micrograms per gram of tissue; the DNA was fully restrictable and was successfully used for sequencing.
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