Special-Base GMCs detection using multiple PCR and pyrosequencing | 基于Special―Base模型检测转基因作物nos／plamid构建特异性
2012
Lan Qingkuo, Tianjin Academy of Agricultural Sciences, Tianiin (China) | Wang Yu, Tianjin Academy of Agricultural Sciences, Tianiin (China) | Zhao Xin, Tianjin Academy of Agricultural Sciences, Tianiin (China)
中国人. 以转基因作物中常见的nos／plamid构建特异性序列为研究对象，通过同源性比较和焦磷酸测序dispensation order设计，建立special-Base焦磷酸测序检测模型；利用转基因大豆RoundupReadySoybean和转基因玉米Btll、GA21、NK603等4种转基因作物为试验材料，针对nos／plamid构建特异性序列设计引物，经复合PCR扩增及焦磷酸测序，验证Special-Base焦磷酸测序检测模型有效性。结果表明：本研究建立的Special-Base焦磷酸测序模型能检测出样品中单一或多种转基因作物成分，提高了转基因作物检测的精确性、检测通量和自动化程度。
显示更多 [+] 显示较少 [-]英语. Despite the various technologies in place for GMC test,a robostic method is highly demanded. The technology described here represents a breakthrough development in that direction. Aimming to nos/plamid gene of four GMCs （RRS,Bt11, GA21, NK603）, by combining the multiple PCR with pyrosequencing, we developed a rapid assay for genotyping nos/plamid that relies on the identification of a single, type-specific ＇sentinel＇ base. The prototype assay was developed to recognize the four GMCs and was capable of recognizing simultaneously multiple GMCs infections. The studies established a rapid, reliable and cost-effective assay.
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