Effect of body condition score on oocyte yield and in vitro embryo development
2012
Kubovicova, E., Vyzkumny Ustav pro Chov Skotu Rapotin, Vikyrovice (Czech Republic) | Makarevic, A.V., Vyzkumny Ustav pro Chov Skotu Rapotin, Vikyrovice (Czech Republic) | Hegeduesova, Z., Vyzkumny Ustav pro Chov Skotu Rapotin, Vikyrovice (Czech Republic) | Slezakova, M., Vyzkumny Ustav pro Chov Skotu Rapotin, Vikyrovice (Czech Republic) | Bezdicek, J., Agrovyzkum Rapotin, Vikyrovice (Czech Republic)
The aim of our study was to evaluate the influence of body condition score (BCS) on oocyte yield and bovine embryo development in vitro. Each of the 163 dairy cows used as a source of ovaries were given a BCS using a scale of 1 (emaciated) to 5 (obese) before slaugther. A total of 326 ovaries from cows with BCS 1-4 were collected. The following ovarian characteristics were evaluated: mean number of follicles, yield and quality of oocytes, embryo development. The mean number of follicles per ovary was significantly lower in cows with BCS 4 (5.19+/-1.24) than in cows with BCS 1 (13.33+/-1.31), BCS 2 (15.33+/-2.06) and BCS 3 (18.04+/-2.66). The quality of oocytes (maturation grade) recovered from cows with BCS 3 (61.27%) was significantly higher than in cows with BCS 4 (35.48 %), BCS 1 (45.27 %) and BCS 2 (48.97 %). In the BCS 3 group the most of matured oocytes (96.13 %) were fertilized. There were no differences in cleavage and blastocyst rate among BCS 1 (50.47 and 24.07 %), BCS 2 (42.7 and 26.7 %) and BCS 3 (46.6 and 24.30 %) categories. Our results indicate that cows tended to emaciation and those tended to obesity have significantly lower prematuration oocyte quality. The oocytes recovered from the cows with average body condition have an optimal oocyte quality suitable for in vitro embryo production. Maturation rate of oocytes and in vitro embryo development are not greatly influenced by BCS, but are rather a function of in vitro conditions.
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