Inhibition of Aflatoxin production by Bacillus subtulis
2009
Saleh, N.A. | Abdel-Aziz, M.S. | Helmy, N.M. | Abdel-Ghany, I.A.
Aflatoxins are toxic metabolites mostly produced by two fungal species (Aspergi)/us flavus and Aspergi//us parasiticus) in cereal grains, groundnuts, corn and rice. In the present investigation the HPLC technique was used for estimation of aflatoxins produced by each Aspergillus species either without or with co-cultivation of B. subtilis. The antitoxin production activity of Bacillus subtilis was detected when being co-cultivated with Aspergillus flavus and Aspergillus parasiticus under different cultural conditions ( pH, temperatures , incubation periods and two types of media). The thermal stability of the antitoxin production element by bacterium was post-heating to 80, 100 °C and autoclaving at 121 °C. Maximum antiaflatoxigenic effect of this bacterium was observed at 25 °C and pH 10 after four days cultivation with Aspergillus flavus, while, the activity appeared at 25 °C and pH 10 after ten days when cultivated with Aspergillus parasiticus. The antitoxin production metabolites are shown to be thermostable at 80, 100°C and autoclaving at 121°C for 30 minutes.
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