Effects of Light Quality and Lighting Type Using an LED Chamber System on Chrysanthemum Growth and Development Cultured In Vitro
2010
Heo, J.W., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Lee, Y.B., Rural Development Administration, Suwon, Republic of Korea | Chang, Y.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Lee, J.T., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Lee, D.B., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea
This experiment was carried out to investigate the effect of light qualities and lighting types provided by LED Chamber System which designed by Rural Development Administration on growth and development of Chrysanthemum (Dendranthema grandiflorum L., cv. 'Cheonsu') plantlet cultured in vitro. The explants of single-node cuttings were exposed to monochromic or mixture radiation of blue, red, or green under continuous and intermittent lighting for 42 days. The intermittent lighting of 20 sec. on and off per minute significantly stimulated shoot elongation with lower number of internodes compared with continuous lighting treatments. However, continuous blue, red, or green light gave greater dry weight comparing the intermittent lighting, and the lowest weight was recorded at the continuous fluorescent lamp. Otherwise, the plantlet growth in dry weight or leaf area was inhibited by the green light controlled at 50 times intermittence but internode elongation was significantly increased. These results showed that the plantlets were successfully grown under the LED Chamber System controlled with different light qualities and lighting types. Quantitative growth of the plantlets was improved under the shorter photoperiod with a intermittent lighting cycle compared with continuous lighting using fluorescent lamps. It is concluded that the growth and development of in vitro plantlets such as single-node cuttings can be achieved by the controlling of light quality or lighting type during the photoperiod per day with a lower electric cost compared with conventional continuous lighting system.
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