Effect of heat and autoclave on allergenicity of porcine serum albumin
2011
Kim, K.B.W.R., Pukyong National University, Busan, Republic of Korea | Lee, S.Y., Korea Food Research Institute, Seongnam, Republic of Korea | Song, E.J., Pukyong National University, Busan, Republic of Korea | Kim, K.E., Yonsei University College of Medicine, Seoul, Republic of Korea | Ahn, D.H., Pukyong National University, Busan, Republic of Korea
This study was performed to investigate the effects of heat and autoclave treatment on the binding ability of porcine serum albumin (PSA) to IgG and the serum of patients who were allergic to pork. Heat treatment at 100℃ for 20 min on PSA was able to decrease the binding ability of PSA to IgG to 52%. The binding ability of PSA to IgG was also reduced to 29% by autoclave treatment of PSA at 121℃ for 30 min. The binding ability of PSA autoclaved for 30 min to serum also decreased to 10%. SDS-PAGE and immunoblotting of PSA heated at 100℃ for 20 min revealed that the intensity of the PSA band decreased and its presence was barely recognized by IgG. In the case of autoclave treatment (for 5 min), PSA was degraded to low-molecular-weight peptides and IgG and serum did not recognize them. Therefore, autoclave treatment can effectively reduce the allergenicity of PSA.
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