Heterologous Expression of a Gibberellin 2-Oxidase Gene from Arabidopsis thaliana Enhanced the Photosynthesis Capacity in Brassica napus L.
2011
Zhou, Bo, Hunan University, Hunan, China | Peng, Dan, Hunan University, Hunan, China | Lin, Jianzhong, Hunan University, Hunan, China | Huang, Xingqun, Hunan University, Hunan, China | Peng, Wusheng, Academy of Seed Industry of Hunan Yahua, Hunan, China | He, Reqing, Hunan University, Hunan, China | Guo, Ming, Hunan University, Hunan, China | Tang, Dongying, Hunan University, Hunan, China | Zhao, Xiaoying, Hunan University, Hunan, China | Liu, Xuanming, Hunan University, Hunan, China
Gibberellins (GAs) are endogenous hormones that play an important role in regulating plant stature by increasing cell division and promoting seed germination. The GA2-oxidase gene from Arabidopsis thaliana (AtGA2ox8) was introduced into Brassica napus L. by Agrobacterium-mediated floral-dip transformation with the aim of decreasing the amount of bioactive GA and hence reduced the plant height. As anticipated, the transgenic plant exhibited dwarf phenotype. Importantly, compared with the wild type, the transgenic plants had delayed the seed germination, increased the chlorophyll content (28.7-36.3%) and photosynthesis capacity (14.3-18.7%) in a single leaf. At the same time, the photosynthesis capacity of the whole plants was significantly enhanced (35.7-48.6%) due to the extra leaves and branches.
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