Molecular Biological Characterization of the First Newcastle Disease Virus Isolated in Mongolia
2011
Choi, K.S., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Lee, E.K., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Jeon, W.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Batchuulon, D., State Central Veterinary Laboratory, Ulaanbaatar, Mongolia | Sodnomdarjaa, R., State Central Veterinary Laboratory, Ulaanbaatar, Mongolia | Park, M.J., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Yoo, Y.N., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea | Kwon, J.H., National Veterinary Research and Quarantine Service, Anyang, Republic of Korea
The outbreak of Newcastle disease occurred for the first time at a commercial chicken farm near Ulaanbaatar, Mongolia in August 2010. Newcastle disease virus (NDV) obtained from infected chickens in Mongolia was characterized by biological and molecular biological approches. Mongolian NDV isolate killed all of chicken embryos within 60 h in the mean death time assay, indicating virulent for chicken. A genomic region of 695 nts between nts 1055 of the M gene and 508 of the F gene was amplified by RT-PCR and sequenced. The deduced amino acid sequence of the F protein cleavage site was ∨112RRQKRF∨117, which is a typical sequence of velogenic strains of NDV and is agreement with the result of the MDT assay. The sequence of the partial F gene (nts 47 to 435) was used for genotyping by phylogenetic analysis. The phylogenetic analysis showed that the Mongolian isolate was of genotype Ⅶ within classⅡ of NDV. Further phylogenetic analysis on the genotype Ⅶ strains revealed that the isolates placed in a genetic sublineage of Ⅶd and most closely related with velogenic strains of NDV circulating in Far-east Asian region especially China, suggesting the introduction of velogenic NDV into Mongolia from neighboring countries.
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