Genome analysis of sugarcane (cultivar BL4) to investigate transposable elements
2011
Raza, S. (Nuclear Inst. of Agriculture, Tandojam (Pakistan)) | Anjum, S. (Karachi Univ. (Pakistan). The Karachi Inst. of Biotechnology and Genetic Engineering) | Qamarunisa, S. (Karachi Univ. (Pakistan). The Karachi Inst. of Biotechnology and Genetic Engineering) | Jamil, I. | Azhar, A. | Qureshi, J.A.
Transposable elements (TEs) are abundant in eukaryotic genomes. About 50-80% of the genomic DNA of grasses is represented by TEs and these elements are responsible for the genome size variations. TEs are considered to have potential involvement in the phenotypic evolution. This study relied on degenerate primers for detection of Tyl-copia elements (Class I TE or retroelement) and designed primers from the conserved sequences for the detection of Mutator (Mu) and Activator (Ac) (Class II TEs) elements. The results obtained through PCR amplification confirmed that all three types of TEs investigated were present in BL4.
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