Response of Artemia franciscana fed with Aeromonas hydrophilla and challenged with Vibrio campbellii
2011
Ali, W. (Pakistan Council of Scientific and Industrial Research Labs., Karachi (Pakistan). Food and Marine Resources Research Centre) | Sultana, R. (Pakistan Council of Scientific and Industrial Research Labs., Karachi (Pakistan). Food and Marine Resources Research Centre)
Gnotobiotically grown Artemia is already proved useful in providing better understanding of host-microbial interactions. The aim of the present study is to make use of gnotobiotically grown Artemia in understanding the effect of Vibrio campbellii on Artemia franciscana fed with Aeromonas hydrophilla. In the first instance, the optimization of V. campbellii concentration was done to establish the challenge dose to Artemia up to 48 h. It was observed that Anemia when challenged with concentration of 10(7) cells /ml of V. campbellii showed lowest survival hence this concentration has been used for the next experiments. In the other part, the optimization of concentration of dead LVS3 as feed was done up to 96 h. Four different concentrations of dead LVS3 were used in the same ratio together while animals having no feed and challenged with V. campbellii were taken as controls. The lowest survival was found with full concentration of LVS3 (10.5x10(9) cells/glass tube).
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