Production of polyclonal antibody against an Indonesian isolate of Chrysanthemum virus B (CVB)
2011
Temajos, I.G.R.M. | Sudana, I.M., Udayana Univ., Bali (Indonesia). Lab. of Phytopathology | Suastika, G., Bogor Agricultural Univ., Bogor (Indonesia). Dept. of Plant Protection
Chrysanthemum Virus B (CVB)is a carlavirus that has been reported as one of the major viruses infecting chrysanthemum worldwide. Leaf mottling or mild vein-clearing in associated with CVB infection, although most of the cultivars are symptomless. The infection may also result in reduced flower quality. In order to produce healthy plants of chrysanthemum demands the screening of chrysanthemum materials for CVB before they can be transported from one country to another. Guidelines for CVB in a certification scheme recommend that CVB infection checked by serology. The success and accuracy of serological techniques to detect and identify viruses is dependent on the availability of diagnostic reagents such as antibody with good quality. The aim of this research is to produce polyclonal antibody of the CVB by guinea-pig immunization using purified CVB of Indonesian isolate. The antiserum was used further for the serological test. Serological methods for detection of CVB were I-ELISA and TBIA. The result showed that guinea-pig immunization using 150ug of purified virus was able to produce 10.75 ml of antibody. The antibody produced had high sensitivity for detection of CVB when examined by I-ELISA and TBIA.
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