Mannanase production and its potent application in feed
2011
Saelee, N., Walailak Univ., Thasala, Nakhonsithammarat, 80160 (Thailand). Dept of Biotechnology
The supplementation of enzymes in the animal feed industry is currently very popular in Thailand. Enzymes promote the digestibility of non-starch polysaccharide which increases the energy and amino acid digestibility. However, the commercial production is very few and the cost of enzyme and feed ingredients has continued to rise due to the increase in demand and cost of raw materials. This research aimed to investigate the optimal conditions and effectiveness of Aspergillus wentii in producing mannanase using Palm Kernel Meal (PKM) as a substrate in Solid State Fermentation (SSF). Without any supplementation, PKM is a good source of substrate for A. wentii to produce mannanase. It secreted mannanase when grown on PKM as an impure inducer. The highest mannanase activity was obtained at 37 U/g koji and 40-60% initial moisture level was suitable for mannanase production. Decreasing the inoculum level prolonged the time for mannanase production in contrast to the medium depth. At higher temperature (35-45 deg C), there is little influence on fungal growth but an obvious decrease in mannanase production was observed. The maximum increase in reducing sugar and crude protein contents of fermented PKM was achieved at 4.5 and 31.6%, gDN, respectively. During mannanase production, low concomitant cellulase and xylanase were also found. These results indicate the efficient induction of an impure PKM for mannanase production by A. wentii. This work revealed the potential of fermented PKM as source of feed additive.
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