Phytochemical analysis and antioxidant properties of Teucrium stocksianum flower from Malakand division, Pakistan
2013
Rahim, G. (Pir Mehr Ali Shah Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Botany) | Qureshi, R. (Pir Mehr Ali Shah Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Botany) | Arshad, M. (Pir Mehr Ali Shah Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Botany) | Gulfraz, M.
The flower extracts of Teucrium stocksianum were screened for antioxidant and phytochemical constituents by using nine different solvents such as acetone, butanol, chloroform, ethyl acetate, ethanol, methanol, n-hexane, petroleum ether and water. By using these extracts, 10 phytoconstituents were screened. Saponins detected by all solvents, followed by tannin, reducing sugar (each 7), flavonoids (6), terpenoid (5), alkaloids, anthraquinone (4 each), whereas, steroids detected by 3 and phlobatannin as well as glycoside isolated by 2 solvents. Three solvents viz., chloroform, ethyl acetate and water extracted highest weight of raw material (150 g), followed by petroleum ether (130 g), n-hexane, acetone (120 g each), methanol (118 g). N-hexane yielded highest extract weight (26 g), followed by butanol (20 g), methanol (19.3 g), water (17 g), acetone (14.5 g), chloroform (13.5 g), petroleum (12.3 g) and ethanol (11.4 g). The percentage yield of extract was varied ranging from 1.73 to 29.08 with the maximum value recorded from n-hexane (21.6%). It was followed by butanol (20.3%), methanol (16.3%), acetone (12.08%), water (11.33%), petroleum ether (9.46%) and chloroform (9%). The plant possessed good antioxidant property and at the dose of 100 mug, DPPH value of flower was 412.82 plus minus 0.003 that is near to the samdard (440.54 plus minus 0.001). The phenolic compounds were also detected in plant that was about half of the quantity of standard (gallic acid). This study will serve as benchmark for further detailed analysis of plant extract prior to drug development and its utilization in future.
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