Cadmium effects on the ratio of activities of lysosomal and total acid phosphatases (ACPlys/ACPtot) in Lymantria dispar larvae
2013
Vlahović, M. (Institute for Biological Research Siniša Stanković, Belgrade (Serbia). Department of Insect Physiology and Biochemistry) | Ilijin, L. (Institute for Biological Research Siniša Stanković, Belgrade (Serbia). Department of Insect Physiology and Biochemistry) | Matić, D. (Institute for Biological Research Siniša Stanković, Belgrade (Serbia). Department of Insect Physiology and Biochemistry) | Lazarević, J. (Institute for Biological Research Siniša Stanković, Belgrade (Serbia). Department of Insect Physiology and Biochemistry) | Nenadović, V. (Institute for Biological Research Siniša Stanković, Belgrade (Serbia). Department of Insect Physiology and Biochemistry) | Perić-Mataruga, Vesna (Institute for Biological Research Siniša Stanković, Belgrade (Serbia). Department of Insect Physiology and Biochemistry) | Mrdaković, Marija (Institute for Biological Research Siniša Stanković, Belgrade (Serbia). Department of Insect Physiology and Biochemistry)
Searching for novel molecular biomarkers, we investigated cadmium effects on the ratio of specific activities of lysosomal and total acid phosphatases (ACPLys/ACPTot) in 4th instar gypsy moth larvae. After acute and chronic exposure to 10 and 30 μg Cd/g dry food, as well as after recovery from both concentrations, the trait values, plasticity, variability and genetic correlations were evaluated. The ACPLys/ACPTot ratio decreased during acute and chronic effects of both concentrations. Inhibition during long-term cadmium exposure was irreversible. Indices of phenotypic plasticity for ACPLys/ACPTot ratio were positive for all cadmium treatments. The variability of plasticity was higher after recovery from 10 μg Cd/g dry food, compared to recovery from 30 μg Cd/g dry food. A significant correlation coefficient was calculated between shortterm cadmium treatments. Significant changes in the ACPLys activity fraction during all treatments indicate the examined trait (ACPLys/ACPTot) could be used as a pollution exposure biomarker.
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