Interlaboratory comparison for determination of ochratoxin A by ELISA in maize (Running title: Determination of ochratoxin A in maize)
2013
Jakšić, Sandra M. (Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)) | Jajić, Igor M. (Faculty of Agriculture, Novi Sad (Serbia)) | Nešić, Ksenija D. (Institute of Veterinary Medicine of Serbia, Belgrade (Serbia)) | Stojanov, Igor M. (Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)) | Živkov-Baloš, Milica M. (Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)) | Mihaljev, Željko A. (Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)) | Abramović, Biljana F. (Faculty of Science, Novi Sad (Serbia). Department of Chemistry, Biochemistry and Environmental Protection)
Participation in interlaboratory comparison and proficiency testing schemes is important for laboratories to control the work quality. In this study, a sample of naturally contaminated maize was analyzed for the content of ochratoxin A (OTA) in three laboratories in Serbia. Participating laboratories used enzymatic immunoaffinity method (ELISA) for the determination of OTA and selection of the ELISA kit was free. Between-laboratory precision was acceptable as evidenced by Cochran’s C test. Moreover, z-scores for all three laboratories were z < ± 2, which is considered acceptable. Used OTA confirmation methods were thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC), with fluorescence detector. The results of different methods were comparable.
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