Development of heme iron feed supplement for prevention and therapy of anemia in domestic animals
2012
Kostić, Ivana T. (Faculty of Technology and Metallurgy, Belgrade (Serbia)) | Stojanović, Radoslava N. (Faculty of Technology and Metallurgy, Belgrade (Serbia)) | Ilić, Vesna Lj. (Institute for Medical Research, Belgrade (Serbia)) | Zarić, Milana M. (Faculty of Technology and Metallurgy, Belgrade (Serbia)) | Đorđević, Verica B. (Faculty of Technology and Metallurgy, Belgrade (Serbia)) | Bugarski, Branko M. (Faculty of Technology and Metallurgy, Belgrade (Serbia))
Animal blood produced in slaughterhouses as a by-product of the meat industry represents serious biohazard. Transformation of wasted slaughterhouse blood to the highly valuable product(s) may partially solve the problem of disposing slaughterhouse blood wastes and at the same time, isolated heme concentrates can be used to fortify feed. The aim of this study was to optimize the isolation process of bovine hemoglobin from slaughterhouse blood by gradual hemolysis in a membrane bioreactor. 35 mM Na-phosphate/NaCl buffer solution of pH 7.2-7.4 was identified as the optimal external medium providing effective gradual osmotic hemolysis with an extent of hemolysis of 88%. The hemoglobin purity of >80% was confirmed by SDS-PAGE. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved, lasted for 90 min. Methemoglobin levels remained below 2% with hemoglobin concentration of 4.8 ±0.5 gL-1 at the end of process. In order to produce ready-to-use feed additive for prevention of iron deficiency anemia in domestic animals, future studies should be oriented toward development of down-stream processes, such as tangential flow filtration and lyophilization.
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