Genetic polymorphism of leptin gene using PCR-RFLP method in three different populations
2012
Ahani Azari, M., Gorgan University of Agricultural Sciences and Natural Resources, Golestan (Iran) | Hasani, S., Gorgan University of Agricultural Sciences and Natural Resources, Golestan (Iran) | Heidari, M., Gorgan University of Agricultural Sciences and Natural Resources, Golestan (Iran) | Yousefi, S., Gorgan University of Agricultural Sciences and Natural Resources, Golestan (Iran)
This investigation was conducted to identify polymorphism of the leptin gene in Holstein cows, Mazandarani native cattle and buffaloes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The frequencies of A and B alleles were 0.4 and 0. 6 - in Holstein cows, 0.56 and 0.44 - in Mazandarani native cattle, 0.61 and 0.35 in buffaloes, respectively. The heterozygote genotype (AB) had the highest frequency in all three populations: 0.73, 0.622 and 0.509 in Holstein cows, Mazandarani cattle and buffaloes, respectively. Chi-square test showed that Mazandarani cattle and buffaloes were in Hardy-Weinberg equilibrium but Holstein cows were not in equilibrium.
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