Verifying of phenotypic stability of pume fruit plants after sanitation procedure
2011
Sedlak, J., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic) | Paprstein, F., Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic)
The aim of this work was to observe, on a long term basis, the phenotypic stability of selected virus free plants of apple Idared and pear Lucasova after thermotherapy. The observation was aimed at 12 (for apple) and 8 (for pear) important characters of shoots and leaves according to classifier for examined fruit species. In the case of three pear clones (22, 49,53) and one apple clone (19 L), any important phenotype differences from control plants were not noted after sanitation procedure. Phenotypic abnormality was observed only in the case of one character (leaf shape) with two clones of Idared (1 and 6) treated by higher temperature 39 deg C. The observed abnormality was incidence of rounded leaves, which are untypical for the cultivar. These untypical leaves, which were noted in the first year in the case of all six plants of Idared clones (1 and 6), grew as the first after transfer to ex vitro onditions. All other leaves, which were formed during the course of vegetation succession and in subsequent years of growing in ex vitro conditions, had all characters identical with the original cultivar. We suppose that temporary incidence of untypical leaves could have been caused by specific in vitro culture conditions (higher humidity and growth regulators 1 used medium) and that it was not mutation.
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