Molecular genetic markers for tissue culture re-sponse in peanut (Arachis hypogaea L.)
Dhuha, S.M. | Taghian, A.S. | El-Aref, H. | Abd El-Fatah, B.E.
Six genotypes of peanut (Arachis hypogeal L.), namely Ismailia-1 (GI),Giza-5 (G2),Agryl (G3), Gregory (G4),Giza-7(G5) and Giza-6(G6) were used for assessing tissue culture response and plant regeneration, genetic variability and polymorphism amongst which and to identify molecular markers associated with tissue culture response using RAPD, ISSR and SRAP techniques, Significant differences were found among the six genotypes for all measured tissue culture traits. The Euclidean-distance dendrogram using tissue culture data separated the six peanut genotypes into two clusters; the first comprised the highly responsive genotypes ( G2,G4 and G5) while the less responsive genotypes (G1,G3 and G6) clustered together in the second. Using three molecular marker systems ( RAPD, ISSR and SRAP ), a total of 202 fragments were amplitied from the six peanut genotypes and 71 (35.15%) of which were polymorphic. The dendrogram generated based on combined RAPD, ISSR and SRAP data showed two main clusters, the first comprised the highly responsive genotypes for tissue culture (G2-G4 and G5 )while the second comprised the three other peanut genotypes ( G1,G3 and G6 which were the less responsive. Highly significant positive correlation ( r = 0.442 : P = 0.001 )was found between the data of the tested molecular markers and tissue culture response. Four DNA fragments [ 461bp (OPA06 ), 1480bp ( HB09), 205bp ( HM12) and 465bp (Emlc-me4)] were found to be positive molecular genetic markers for tissue culture response in peanut genotypes. The investigation demonstrated that RAPD, ISSR and SRAP analyses showed considerable for variety identification and discrimination and could be useful for tissue culture response in peanut.
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