Identification of the F-1-ATPase at the Cell Surface of Colonic Epithelial Cells ROLE IN MEDIATING CELL PROLIFERATION
2012
Huc-Lemarié , Laurence(collaborateur) (INRA , St-Martin-Du-Touch (France). UMR 1331 Toxicologie Alimentaire) | Seva , C(auteur de correspondance) (Université Toulouse 3, 31052 Toulouse(FR). Canc Res Ctr Toulouse, INSERM UMR 1037)
F-1 domain of F1Fo-ATPase was initially believed to be strictly expressed in the mitochondrial membrane. Interestingly, recent reports have shown that the F-1 complex can serve as a cell surface receptor for apparently unrelated ligands. Here we show for the first time the presence of the F-1-ATPase at the cell surface of normal or cancerous colonic epithelial cells. Using surface plasmon resonance technology and mass spectrometry, we identified a peptide hormone product of the gastrin gene (glycine-extended gastrin (G-gly)) as a new ligand for the F-1-ATPase. By molecular modeling, we identified the motif in the peptide sequence (E(E/D)XY), that directly interacts with the F-1-ATPase and the amino acids in the F-1-ATPase that bind this motif. Replacement of the Glu-9 residue by an alanine in the E(E/D)XY motif resulted in a strong decrease of G-gly binding to the F-1-ATPase and the loss of its biological activity. In addition we demonstrated that F-1-ATPase mediates the growth effects of the peptide. Indeed, blocking F-1-ATPase activity decreases G-glyinduced cell growth. The mechanism likely involves ADP production by the membrane F-1-ATPase, which is induced by G-gly. These results suggest an important contribution of cell surface F-1-ATPase in the pro-proliferative action of this gastrointestinal peptide.
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