Arabidopsis formin AtFH6 is a plasma membrane–associated protein upregulated in giant cells induced by parasitic nematodes
2004
Favery , Bruno (INRA , SOPHIA ANTIPOLIS CEDEX (France). UMR 1301 UMR INRA / CNRS / Univ. Nice : Interactions Biotiques en Santé Végétale) | Chelysheva , Liudmila (INRA , SOPHIA ANTIPOLIS CEDEX (France). UMR 1301 UMR INRA / CNRS / Univ. Nice : Interactions Biotiques en Santé Végétale) | Lebris , Manuel (INRA , SOPHIA ANTIPOLIS CEDEX (France). UMR 1301 UMR INRA / CNRS / Univ. Nice : Interactions Biotiques en Santé Végétale) | Jammes , Fabien (INRA , SOPHIA ANTIPOLIS CEDEX (France). UMR 1301 UMR INRA / CNRS / Univ. Nice : Interactions Biotiques en Santé Végétale) | Marmagne , Anne (Centre National de la Recherche Scientifique, Gif sur Yvette(France). Institut des Sciences Végétales) | De Almeida , Janice (INRA , SOPHIA ANTIPOLIS CEDEX (France). UMR 1301 UMR INRA / CNRS / Univ. Nice : Interactions Biotiques en Santé Végétale) | Lecomte , Philippe (INRA , SOPHIA ANTIPOLIS CEDEX (France). UMR 1301 UMR INRA / CNRS / Univ. Nice : Interactions Biotiques en Santé Végétale) | Vaury , Chantal (Institut National de la Santé et de la Recherche Médicale, Clermont-Ferrand cedex(France). Unité 384) | Arkowitz , Robert A. (Centre National de la Recherche ScientifiqueUniversité de Nice Sophia-Antipolis, NiceNice(France). Unité Mixte de Recherche 6543Faculté des Sciences) | Abad , Pierre (INRA , SOPHIA ANTIPOLIS CEDEX (France). UMR 1301 UMR INRA / CNRS / Univ. Nice : Interactions Biotiques en Santé Végétale)
Plant-parasitic nematodes Meloidogyne spp induce an elaborate permanent feeding site characterized by the redifferentiation of root cells into multinucleate and hypertrophied giant cells. We have isolated by a promoter trap strategy an Arabidopsis thaliana formin gene, AtFH6, which is upregulated during giant cell formation. Formins are actin-nucleating proteins that stimulate de novo polymerization of actin filaments. We show here that three type-I formins were upregulated in giant cells and that the AtFH6 protein was anchored to the plasma membrane and uniformly distributed. Suppression of the budding defect of the Saccharomyces cerevisiae bni1Δ bnr1Δ mutant showed that AtFH6 regulates polarized growth by controlling the assembly of actin cables. Our results suggest that AtFH6 might be involved in the isotropic growth of hypertrophied feeding cells via the reorganization of the actin cytoskeleton. The actin cables would serve as tracks for vesicle trafficking needed for extensive plasma membrane and cell wall biogenesis. Therefore, determining how plant parasitic nematodes modify root cells into giant cells represents an attractive system to identify genes that regulate cell growth and morphogenesis
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