Valorisation of genetic and genomic resources of tomato for the improvement of fruit quality
2012
Causse , Mathilde(auteur de correspondance) (INRA , Montfavet (France). UR 1052 Génétique et Amélioration des Fruits et Légumes) | Brunel , Dominique (INRA , Evry (France). US 1279 Etude du Polymorphisme des Génomes Végétaux) | Amouroux , P. (Vilmarin & Cie, Chappes(France).)
ln this project we intend to develop the resources necessary for high throughput QTL studies in tomato bycombining the development of genomic and plant resources including:(1) a large set of SNPs in expressed sequences and flanking regions based on high throughput sequencingtechnology.(2) a set of 4 Illumina VeraCode chips carrying a total of 1536 SNP(3) Creation and phenotype characterisation of a multiparent advanced generation intercross (MAGIC) populationto be used for QTL mapping and latter for fine mapping, as a complement to genetic resources gathered incollections. The population will be characterised for yield related traits and fruit quality (composition in sugars and acids, colour, texture).(4) Differentiai Gene Expression profiles and proteome profiles of the 8 parental lines of the MAGIC populationsand the 4 Fl hybridsThese resources are developed to be used in the subsequent analyses:(1) Genotyping a biallelic population in order to precisely map ail the markers present on the VeraCode chips(2) Genotypinç the MAGIC population with the Illumina VeraCode chips in order to map QTL(3) Genotyping several genetic resources (collections of cherry tomato cultivars, of old cultivars and a set of elitegenotypes), in order to assess linkage disequilibrium and search for associations between SNP markers and fruitrelated traits(4) Expression profile analysis of the genes and proteins characterised at the 2 stages of expression; comparison among genotypes and Fl hybrids. The final expected result is a genetic map of more than 1000 SNPs which will be associated with QTL locations and expression variations of the parental lines and Fl hybrids.
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