Comparison of the validity of CIE and PCR diagnostic methods in determining hydatidosis in sheep
2012
Yakhchali, M., Associate Professor, Department of Pathobiology, Parasitology Division, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | Morshedi, A., Associate Professor, Immunology Division, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | Shirashian, M., General Practitioner of Veterinary Medicine, Urmia, Iran
Sheep is one of the important hosts for hydatidosis in Iran as a hyperendemic region worldwide. In the present study, the efficacy of counter immuno-electerophoresis (CIE) and PCR tests to detect sheep hydatid cyst infection was carried out. For this, soluble antigen was provided using hydatid cyst fluids (HCFs). Blood sera were taken from a total number of 100 slaughtered sheep. CIE analysis was undertaken using negative and positive controls. DNA was extracted from protoscolices and a 1213bp fragment length of co1 gene amplified. Necropsy findings indicated that lung and liver of slaughtered sheep (33%) was infected with hydatid cyst. While it was 29% in CIE and 28% in PCR test. According to the necropsy findings as a gold standard test, sensitivity and specificity of CIE was 79.31% and 92.2%, respectively. Sensitivity and specifity of PCR test were determined 85.19% and 94.8%, as well. Data analysis showed that there is no significant difference between these two techniques (P0.05). Based on these results, CIE technique can be considered as a useful method to screen sheep hydatid cyst infection.
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