Effect of dietary protein and energy levels on growth of juvenile orange-spotted grouperâÂÂ, Epinephelus coioidesâÂÂ
2011
Ghafleh Marammazi, J., South Aquaculture Research Center- Ahvaz | Zabayeh Najafabadi, M., South Aquaculture Research Center- Ahvaz | Pagheh, E., South Aquaculture Research Center- Ahvaz | Ahmadi, B., Khoramshahr Marine Science & Technology university | Eskandari, GH., South Aquaculture Research Center- Ahvaz | Hafezieh, M, Iranian Fisheries Research Organization
In order to evaluate the protein and energy requirement of orange-spotted grouperÃÂ Epinephelus coioides fingerlingsÃÂ nine semi-purified experimental diets containing 3 levels of crude protein (40%ÃÂ 50% and 60%) and 3 levels of diegestible energy (DEÃÂ 14ÃÂ 16 and 18 KJgr-1)ÃÂ in a flow-throught system (2 Litmin-1) at 24.32 1.11 C were tested. For 8-weeksÃÂ Triplicate groups of 20 fish (16.79 .4 gr) were each stocked in 300 L circular polyethylene tanks and were fed the experimental diets to approximate satiationÃÂ twice daily. Results showed that the survival rateÃÂ hepatosomatic index (HSI) and condition factor (CF) were independent of the dietary treatmentsÃÂ but final weightÃÂ weight gainÃÂ specific growth rate(SGR)ÃÂ feed efficiency (FE)ÃÂ feed conversion ratio (FCR)ÃÂ protein efficiency ratio (PER) and daily feed intake (DFI) were significantly affected by protein and digestible energy levels. Diet 5 (50%CP and 16 KJ/gr DE) was the preferred diet and resulted in the best growth performanceÃÂ feed and protein efficiency and nutrient utilization among the examined diets. DFI was the lowest for diet with the highest protein (60%) and energy (18 KJgr-1). Apparent net protein utilization (ANPU) varied insignificantly (P0.05) between the treatments. viscerosomatic index(VSI) increased significantly with increasing dietary energy concentrations. Body lipidÃÂ ash and moisture contents were affected by dietary protein and energy levels (P0.05). Body lipid content increased with increasing dietary energy levelsÃÂ but carcass ash and moisture decreased with increasing dietary energy levels. Body protein content was not affected by dietary energy levelÃÂ but significantly increased with the increase of protein from 40 to 50% at same energy levels. The study revealed that fingerlings of orange-spotted grouperÃÂ E. coioides performed best a diet containing 50% crude proteinÃÂ 16 KJgr-1 diegestible energy and a P/DE ratioÃÂ 31.25 mgkJ-1. Key words: Epinephelus coioidesÃÂ proteinÃÂ energyÃÂ growthÃÂ feed utilizationÃÂ body composition
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