Function and composition of microbiol community in the animal waste treatment
2004
Nakai, Y. | Wakasle, S. | Otawa, K. | Kitazume, O. | Nonaka, J. | Satoh, M. | Sasaki, T. | Sasaki, H. | Ito, K.
We observed effects of a commercial microbiological additive (MA) to the beef manure composting process, and investigated microbial communities in MA itself and the chicken manure composting process added with MA by the conventional cultivation method and the advanced DNA sequencing analysis. When we inoculated beef manure with MA, we found quicker elevation of temperature at the start of composting and after the first turning, lower emission of ammonia gas, lower production of nitrate, larger numbers of mesophilic aerobes, thermophilic aerobes and anaerobes, and smaller number of thermophilic aerobes, compared with the composting process without MA. These results suggested that mesophilic aerobes increased in number and metabolized ammonia assimilatively rather than nitricatively to accelerate the temperature elevation of the compost by the inoculation of MA. Functional microbes obtained by the cultivation method from MA were not coincided with dominant species in the microbial community detected by DNA analyses. Various species of microbes in MA grew at 55 and 72C incubation but they were not coincided with dominant species detected from composting processes. It was clarified that MA contained variety of microbes including thermophilic microbes, and that these microbes did not become dominant in the composting process. Microbes in MA that were smaller in number than dominant species may act as functional microbes in the compost process.
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