Callus induction in cotyledons of Moringa oleifera Lam.
2011
Nieves, M.C., Central Luzon State Univ., Science City of Muñoz, Nueva Ecija (Philippines) | Aspuria, E.T., Philippines Univ. Los Banos. College, Laguna (Philippines). Crop Science Cluster
Callus cultures of Moringa oleifera Lam. were successfully established from cotyledon explants obtained from green mature seeds. Benzyl adenine (BA) alone (2.5 mg/L) significantly increased callus formation but was not significantly different when combined with increasing levels of 2,4 dichlorophenoxy acetic acid (2,4-D) (1.0-5.0 mg/L) and further supplemented with thidiazuron (TDZ). Loose crystalline calli were observed in media containing 2,4-D and BA singly or in combination, while compact, modular and loose calli were obtained when the culture media were further enriched with TDZ. BA and 2,4-D singly or in combination did not significantly enhance degree of callus formation but BA in combination with 1.0 and 5.0 mg/L 2,4-D and TDZ significantly increased degree of callusing. A significant increase in callus weight was observed in BA at different levels combined with 2,4-D (1.0 and 2.5 mg/L). Addition of TDZ to BA and 2,4-D containing media further increased callus weight. Slight greening of calli was observed 4 wk after the first transfer onto the shoot induction media, however, statistical analysis revealed that there was no significant difference among treatments. Microscopic observation of hand-sectioned protrusions on green calli revealed heterogeneous and asynchronous cell growth showing actively divided cells. Xylem differentiation was also evident, indicating initiation of bud initials.
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