Proliferation and regeneration protocol for protocorm-like bodies of Phalaenopsis gigantea orchid.
2011
Suryanti, B. | Sinniah, U.R., Universiti Putra Malaysia, 43400 Serdang , Selangor (Malaysia). Dept. of Crop Science | Subramaniam, S., Universiti Sains Malaysia, Minden Heights, 11800, Penang (Malaysia). School of Biological Sciences
Phalaenopsis is one of the popular orchids which has long arching sprays, beautiful flower shelf-life. One of the important Phalaenopsis species is Phalaenopsis gigantea which is highly sought after by the orchid collector. Mass production of phalaenopsis is via micropropagation through the protocorm-like bodies which is a rapid method of proliferation within a short period of time. Each PLBs can be regenerated into individual plantlets when cultured into media (Park et.al., 2000). This study was done to determine the suitable type of culture media for the PLBs proliferation. The type of media tested was liquid and solid New Dogashima media supplemented with 1mg/L NAA and 0.1 mg/L TD2. Four PLBs were inoculated into 100mL conical flasks. Besides, different size of PLBs namely 1,2 and 3 mm were cultured onto the hormone free NDM media to see the effect of the size on the regeneration process. PLBs cultured in both liquid media as well as solid media did not differ much in their response to proliferation. However, phalaenopsis gigantea appears to exudates a high amount of phenolic compounds which was deleterious in the liquid culture as the spread was quick. At the end of the sixth week the number of PLBs in solid media exceeded the number of PLBs on the liquid media which were 13 and 8, respectively. In both cases, the proliferation was relatively slow as compared to many other orchid species. So, it is suggested that solid media is more appropriate for the proliferation process. In the second part of the study, it was found that although the 3mm PLBs were able to convert into shoot very early but plantlet development was low. PLBs with the size of 1mm had a high rate of conversion into complete plantlet.
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