X-ray flouresence spectrometry (XRF) as a new tool for high throughput screening for zinc content in rice grains
2013
Samia, M. | Asilo, M.A. | Virk, P. | Lopena, V. | Shim, J.H. | Barry, G. | Rey, J.
Malnutrition and hidden hunger due to micronutrient deficiency is known to be a serious problem among children and women, most especially in developing countries in Asia. One sustainable and effective approach to solve this hidden hunger is through breeding for micronutrient-dense staple crops using conventional breeding strategies or modern biotechnological tools commonly known as biofortification. In biofortification breeding programs, screening for genotypes with high zinc will require reliable and cost effective methods to easily identify potential donor lines. Hence, this study aims to evaluate the use of a non destructive X-ray flourescence spectrometry (XRF) method as a reliable tool for high throughput screening for quantifying zinc in the grain: this evaluation compares the XRF method with the current reliable method for elemental analysis, inductively coupled plasma-optical emission spectrometry (ICP-OES). A total of 77 rice genotypes from mapping populations, recombinant inbred line, and elite breeding lines were obtained from existing biofortification programs. Brown rice and milled rice were evaluated for zinc content by ICP-OES and XRF. XRF reading shows bias compared to ICP but based on comparison of regression equation with 1:1 regression line gives high correlation both in brown and milled rice with r square = 0.90 and 0.94, respectively. Thus XRF can be a valuable tool for high throughput screening in probing for high zinc rice lines for biofortification breeding programs. The methods were also compared on the basis of sample size and number of samples handled per day.
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