Improved indirect ELISA for detection of some plant viruses
2013
Fegla, G. (Alexandria Univ. (Egypt). Dept. of Plant Pathology) | Kawanna, M. (Jazan Univ., Jazan (Saudi Arabia). Dept. of Biology)
Addition of 0.1% Triton X-100 improved detection of Bean yellow mosaic virus (BYMV) by indirect ELISA, which was further, increased the absorbance value by 35.04%, when Triton X-100 was added to carbonate buffer. Detailed studies with other three viruses; Cucumber mosaic virus (CMV), Tomato mosaic virus (ToMV), Potato virus Y (PVY) using three concentrations of cellulase, macerozyme or Triton X-100 and two extraction buffers (phosphate or carbonate buffer) were achieved. The absorbance values of CMV, ToMV or PVY infected sap were increased significantly by adding each of the additives tested to both extraction buffers. The most efficient additive was macerozyme when used at 0.1%, since the percent increase in absorbance values of CMV, ToMV and PVY was 40.91, 28.33 and 37.02, respectively when the samples extracted in phosphate buffer. While the percent increase was 40.06, 39.16 and 45.51, respectively when the samples extracted in carbonate buffer. Extraction of CMV, ToMV and PVY infected samples in carbonate buffer increased the absorbance values as compared with phosphate buffer. The maximum increases in absorbance values were 30.89, 29.19 followed by 27.32%, which obtained when PVY infected samples were extracted in carbonate buffer with Triton X-100 (0.25%), (0.1%) and macerozyme (0.1%), respectively.
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