Efficiency of titanium dioxide on mungbean seed sterile and their nanotoxicity to mungbean growth in vitro
2013
Theerakarunwong, C. (Nakhonsawan Rajabhat Univ., Nakhonsawan (Thailand). Dept. of Science) | Chouychai, W. (Nakhonsawan Rajabhat Univ., Nakhonsawan (Thailand). Dept. of Science)
Titanium dioxide (TiO2) nanoparticles have been reported to be microbial disinfectant. Surface sterilization of explants with nanoparticles instead of high toxicity compound, such as, NaOCl, and HgCl2, will be a challenge. TiO2 nanoparticle was synthesized with sol-gel technique. This TiO2 was tested their efficiency to sterilize mungbean seed surface at different concentration and their toxicity to mungbean seedling growth in vitro. There efficiency were compared with standard method used sodium hypochlorite in 10% Hyter (copy right) solution. Sterilized seeds were culture in 1/2 Murashigi and Skoog (MS) media for 10 d. Contaminated seed or seedlings with fungi or bacteria were counted. The result showed that the specific surface area of synthetic anatase TiO2 contained 85.10 m2/g with the average size of 15-30 nm. Only percentage of contamination of seeds sterilized with 50 mg/L TiO2 induced with ultraviolet for 30 min was not significantly different to standard method. The mungbean seedling exposure to 250 - 2,500 mg/L TiO2 in 1/2 MS, both of non-induce and 30 min UV-induced TiO2, did not affect shoot and root growth, and chlorophyll content of 7-day old mungbean seedlings.
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