Production process of extracellular lipases by Fusarium sp. using agricultural byproducts
2012
Iftikhar, T. (Government Coll. Univ., Faisalabad (Pakistan). Dept. of Botany) | Niaz, M. (Government Coll. Univ., Faisalabad (Pakistan). Dept. of Botany) | Ali, E.A. (Government Coll. Univ., Faisalabad (Pakistan). Dept. of Botany) | Jabeen, R. (Sardar Bahadur Khan Univ., Quetta (Pakistan). Dept. of Botany) | Abdullah, R. (Lahore Coll. for Women Univ., Lahore (Pakistan). Dept. of Biotechnology and Microbiology)
The present study piece of study is designed to optimize the environmental conditions for the production of extracellular lipases by Fusarium sp. The fungal culture was checked in order to test its potential for lipases biosynthesis. Almond meal was optimized to be the ideal basal support for the enzyme production using solid state fermentation technique. Ten grams of almond meal supported maximum extracellular lipases (3.48 plus minus 0.01a U/mL) production. Other cultural conditions like level of inoculum, quantity of moistening agent, rate of fermentation, fermentation temperature, nitrogen supplements, carbon additives, addition of various oils were also optimized. One milliliter inoculum, 10mL of moistening agent, 30 degree C incubation temperature, 1% peptone as additional organic nitrogen supplement, 1% Glucose and 1% olive oil was optimized as the ideal parameters for the maximum production of the extracellular lipases. Maximum units of the extracellular lipases (56.7 plus minus 0.01a U/mL) was obtained when incubation period was optimized at 72h.
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