Molecular fingerprint of Ganoderma spp. from Sabah, Malaysia
2013
Khim-Phin, C. (Universiti Malaysia Sabah (Malaysia). Sustainable Palm Oil Research Unit) | Abdullah, S. (Universiti Malaysia Sabah (Malaysia). Sustainable Palm Oil Research Unit) | Tong-Leong, N. (Universiti Malaysia Sabah (Malaysia). School of Sustainable Agriculture)
Basal Stem Rot (BSR) of oil palm (OP) (Elaeis guineensis) caused by Ganoderma boninense is the most devastating disease in South East Asia. High incidence of BSR results in economic losses, due to zero yield from dead palms and significantly reduced weight and number of fruit bunches from infected living palms. Although BSR is a very important disease in OP, molecular information on Ganoderma isolates from Sabah, however is not well documented. There is limited information on the use of molecular techniques in identification of G. boninense available in current literature for G. boninense isolates from Sabah. The only report on this pathogen isolate in Sabah is based solely on their morphology and pathogenicity. In conjunction with the morphological similarities between the different isolates, there are also varied opinions on the aggressiveness of the pathogen in Sabah. In this paper we report on the identity of Ganoderma isolates from several OP Estates in Sabah, Malaysia. The identities of these isolates were confirmed using DNA sequence analysis after PCR amplification. The latter method showed that the Sabah isolates were very similar to Ganoderma sp with a maximum similarity of 99%.
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