In-vitro antibacterial activity of Parthenium hysterophorus against isolated bacterial species from discolored rice grains
2013
Ashfaq, M. (Punjab Univ., Lahore (Pakistan). Inst. of Agricultural Sciences) | Ali, A. (Punjab Univ., Lahore (Pakistan). Inst. of Agricultural Sciences) | Siddique, S. (Punjab Univ., Lahore (Pakistan). Inst. of Agricultural Sciences) | Haider, M.S. (Punjab Univ., Lahore (Pakistan). Inst. of Agricultural Sciences) | Ali, M. (Punjab Univ., Lahore (Pakistan). Inst. of Agricultural Sciences) | Hussain, S.B. (Bahauddin Zakariya Univ., Multan (Pakistan). Coll. of Agriculture) | Mubashar, U. (Elementary Teachers Education Coll., Gujranwala (Pakistan)) | Khan, A.U.A. (Government Coll. Univ., Lahore (Pakistan). Dept. of Zoology)
The frequency of bacterial population was estimated in discolored rice grains of seven different local and exotic varieties. Twelve bacterial species were isolated on Nutrient agar (N.A.) medium and maximum frequency among bacteria (19.32%) observed in KSK-133 and minimum (11.96%) in 140-4-1-2-5 among all rice varieties. Burkholderia pseudomallai and Burkholderia glumae had higher frequency almost in all tested rice varieties, while Acidovorax facilis had higher frequency only in CB- 38. Different extracts/solvents (benzene, ether and chloroform etc) were used to check the antibacterial activity on each rice variety. The aqueous extract showed evidence of low antibacterial activity as compared to other solvents. The maximum inhibition in vitro, was observed in benzene and ether solvent (up to 3.0-3.7 cm) against B. glumae, B. pseudomallei, K. oxytoca, K. sibirica and M. rhodesianum followed by chloroform extract with inhibition zone of 3.0 cm and 3.7 cm against B. glumae and K. oxytoca, respectively. Among different treatments, minimum inhibitory concentration (MIC) varied among 0.25-4.0 mg/mL when tested with all four solvent extracts of P. hysterophorus against rice pathogens. The main objective of the study was to minimize the bacterial diseases by using different extracts/solvents and to increase the high yield potential. The present investigation strongly indicated antibacterial potential of leaf extracts of P. hysterophorus against bacterial pathogens of rice in sustainable disease management and also helpful for increasing the yield of rice crop.
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