Genotypes, explants and growth hormones influence the morphogenesis in Pakistani sorghum (Sorghum bicolour): Preliminary field evaluation of sorghum somaclones
2013
Zarif, M. (University of Agriculture, Faisalabad (Pakistan). Centre of Agricultural Biochemistry and Biotechnology) | Sadia, B. (University of Agriculture, Faisalabad (Pakistan). Centre of Agricultural Biochemistry and Biotechnology) | Kainth, R.A. (Ayub Agricultural Research Inst., Faisalabad (Pakistan). Fodder Research Substation) | Khan, I.A.
Present study reports the establishment of a robust in vitro regeneration protocol for Pakistani sorghum lines, Pak China-1 and Sandal Bar. Mature seeds and immature inflorescences were assessed for morphogenesis. MS medium with 2, 4-D and kinetin induced callus from both the explants. Whereas, MS fortified with NAA (0.5 mg/L) + kinetin (1 mg/ L) + Casein hydrolysate (400 mg/ L) proved the best regeneration medium for immature inflorescence-derived calli. In vitro regeneration from seed-derived calli was the maximum on LS medium supplemented with IAA (2 mg/ L) + BA (2 mg/ L). The axenic regeneration and establishment of sorghum somaclones in the glasshouse completed within 20-22 weeks. Two hundred somaclones have been shifted to the field; their preliminary evaluation indicated variations for some economically important traits including no. of tillers, seed yield, leaf area and stem thickness. Further evaluations are underway.
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