Evaluation of a saponin adjuvanted inactivated Mycoplasma bovis (a field isolate from cattle lungs in Balochistan, Pakistan) vaccine
2013
Ahmad, Z. (University of Balochistan, Quetta (Pakistan). Centre for Advance Studies in Vaccinology and Biotechnology) | Amin, S. (Government of Balochistan, Quetta (Pakistan). Livestock Dept.) | Yasir, M. (University of Balochistan, Quetta (Pakistan). Dept. of Microbiology) | Babar, S. | Abbas, F. | Awan, M.A. | Attique, A. | Khan, M.A. | Rashid, N. | Wadood, A. | Shafee, M. | Asadullah | Jan, S.
An inactivated saponin adjuvanted vaccine was prepared from Mycoplasma bovis local field isolate and its efficacy was evaluated. Nine calves (n=9) were split in three groups (three calves in each group). Calves in group A were vaccinated with M. bovis saponin adjuvanted (inactivated) vaccine and challenged with live M. bovis strain by nasal spray at day 21 post vaccination. Calves in group B were only challenged with live M. bovis culture through same route and Group C was kept as control. All groups of calves were monitored for 7 weeks. The antibody profile of all vaccinated and challenged animal were assessed through IHA test. The saponated M. bovis (inactivated) vaccines with protein concentration (2 mg/mL) was found very effective. Any pathological lesion, mortality and any other clinical manifestation was not observed in vaccinated group of calves. Over all the immune status with a GMT (40.3) was found satisfactory with this vaccine, which was achieved 3 weeks post-vaccination and this titer has risen to a GMT (80.6) after four week and was maintained to a GMT of (64.0) on 49th days at the end of experiment.
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