Study on separation and purification of ginkgo flavone aglycone by macroporous adsorption resin | 大孔树脂分离纯化银杏黄酮苷元的研究
2013
Ma Chaoyang, Jiangnan University, Wuxi (China), School of Food Science and Technology | Lu Wenping, Jiangnan University, Wuxi (China), School of Food Science and Technology | Lou Zaixing, Jiangnan University, Wuxi (China), School of Food Science and Technology
中国人. 以银杏提取物的酶水解液为实验材料，研究大孔吸附树脂分离纯化其中的银杏黄酮苷元的工艺。通过静态吸附一解吸和吸附动力学实验，从3种大孔吸附树脂中筛选出非极性YWD07a为最适合分离纯化银杏黄酮苷元的树脂。YWD07a树脂吸附分离银杏黄酮苷元的最佳工艺条件为：吸附：银杏黄酮苷元的浓度3.26mg/mL，pH5.0，流速2BV/h；解吸：洗脱体积6BV，流速3.0BV/h，乙醇浓度70%。在最优条件下得到的银杏黄酮苷元产品中银杏黄酮苷元含量由18.5%提高到80.5%，回收率为73.6%。
显示更多 [+] 显示较少 [-]英语. In this study, the separation effect of macroporous resin for the purification of ginkgo flavonol aglycone prepared by the enzymatic hydrolysis of the Immobilized B-glucosidase was developed. Based on the results of static adsorption and desorption, adsorption dynamic, YWDO7a resin was the best one for the separation and enrichment of flavonol aglycone. The optimal separation conditions were as follow: on adsorption, flavone aglycone concentration of sample solution, pH, flow rate was 3.3 mg/mL, 5.0, 2 bed volume (BV) per hour, respectively.; on desorption, volume of elution solvent, flow rate, enthaol concentration was 6 BV, 3 BV/h, 70% (v/v), respectively. Under these conditions, the content of flavone aglycone increased from 18.5% to 80.5% in the crude oroduct, and the recovery of flavone aglycone reached 73.6%.
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