Growth Characteristics of Oxyrrhis marina and Chattonella marina in their Co-culture Systems

This study was aimed to investigate the growth characteristics of Oxyrrhis marina and Chattonella marina in co-culture to provide experimental evidences for discussing successions of harmful algal blooms (HABs) and coastal biological communities. The colour changes of culture media of C. marina and growth characteristics of O. marina and C. marina in co-culture were analysed by the combined methods of macroobservation, microscopic examination and counting. In co-culture, the colours of culture media of C. marina had changed and their transparencies had increased with increasing elapsed incubation time after inoculated by O. marina under different initial cell densities. With the increase of the initial density of O. marina (0.17×104 cells/mL, 0.50×104 cells/mL and 0.64×104 cells/mL in C. marina culture media), the time required, that the populations of O. marina reached the stationary phases, was shorter i.e. 6d, 5d and 3d after inoculated by O. marina, respectively, and the death time of all cells of C. marina became shorter, i.e. 7d, 6d and 4d after inoculated by O. marina, respectively. During the 15 days culture period, all C. marina populations were evolved to O. marina populations. Residues of C. marina adhering to precipitates and chromatophores scattering in the culture media could strengthen the colour of culture media, C. marina populations were evolved to O. marina populations respectively within the concentrations designed in co-culture in this experiment. Disturbance feeding was one of the reasons for successions, and the results provide experimental evidences for discussing successions of red tides and coastal biological communities.