Developing and optimizing a molecular diagnosis system for
2013
Pourrahim, Reza, Iranian Research Institute of Plant Protection | Farzadfar, Shirin
Potato virus A is one of the most important viruses infecting potato in the world. During spring and summer of 2011 and 2012, a total of 395 and 1249 symptomatic and random leaf samples, respectively were collected from potato fields in Ardebil, Hamedan, Isfahan, and Kerman provinces and tested for PVA infection using DAS-ELISA method. Based on the results, PVA infection was recorded in 27.3 and 11.1 % of symptomatic and randome leaf samples. Biological properties of selected PVA isolates were studied using host range studies on 10 plant species. Eight PVA isolates were selected and their coat protein (CP) gene was amplified by specific primers using RT-PCR and their nucleotide sequences were determined (KF179231-8). These sequences together with the CP sequences of four other Iranian and 37 Non-Iranina PVA isolates were compared and a phylogenectic tree was reconstructed using Neighbor Joining-NJ method with 1000 bootstrap value. The PVA isolates in the phylogenic tree were clustered in two main groups (I and II) which group I had two subgroups (IA and IB). All eight Iranian PVA isolates (except for Jir11) were grouped in IA subgroup with highest similarity with two isolates from Germany (Y11426) and Netherlands (X91968) and the other Iranian PVA isolates (Jir11 and Gh4 from Jiroft and Khorasan-e- Razavi provinces, respectively) were grouped in IB subgroup showing the highest similarity with isolates from the Netherlands (Y10126 and Y11422). This is the first phylogenetic study on Iranian PVA isolates in the surveyed provinces. This study showed variation among PVA isolates in Iran. Thses data have to be concidered in potato breeding programes for achiving PVA resistant cultivars.
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