Comparison of two methods to measure nitrate in plant tissue
2013
Feizolahzadeh, M. | Bazargan, K. | Tabakhian, Sh | Sagafi, K | Karimion, M.H
Routine laboratory method to measure nitrate in plants is diazo. Because of the many problems that have been reported in the procedure of this method, we decided to evaluate sulphosalicylic method as an alternative method. Therefore, about 50 samples such as potato tubers, some vegetables, hay, maize and rice were used for comparison. The plant samples after preparation(washing, drying, grinding, sieving) were measured by two methods at two replicates. A randomized complete block factorial was run with two replicates and two factors (methods &type of plant) by Minitab, Excel and MSTATC software. The results of the analysis showed that the two methods had a significant difference at 5% statistical level. Diagram of nitrate concentration differences in between the two methods that was plotted against the concentration of nitrate in the Common method, showed in plants with less than 1,500 mgkg-1, nitrate Concentrations in sulphosalicylic method may be reported up to 10 times more than the common method. A separate analysis of samples with high concentrations(more than 1500 mg kg-1 of nitrate wasn't observed a nonsignificant difference at 1% statistical level. Because of the simplicity, repeatability and speed work, sulphosalicylic method recommended this method use for the determination of nitrate in leafy vegetables with high nitrate levels. Due to the low concentration of nitrate in tubers and seed plants such as potatoes, corn and rice will avoid from using this method.
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