A Novel Marker for the Species-Specific Detection and Quantitation of Vibrio cholerae by Targeting an Outer Membrane Lipoprotein lolB Gene
2013
Cho, M.S., RDA, Suwon, Republic of Korea | Ahn, T.Y., Dankook University, Cheonan, Republic of Korea | Joh, K., Hankuk University of Foreign Studies, Yongin, Republic of Korea | Paik, S.Y., The Catholic University of Korea, Seoul, Republic of Korea | Kwon, O.S., National Institute of Environmental Research Environmental Research Complex, Incheon, Republic of Korea | Jheong, W.H., National Institute of Environmental Research Environmental Research Complex, Incheon, Republic of Korea | Joung, Y.C., Hankuk University of Foreign Studies, Yongin, Republic of Korea | Park, D.S., RDA, Suwon, Republic of Korea
Vibrio cholerae O1 and O139 are the major serotypes associated with illness, and some V. cholera non-O1 and non-O139 isolates produce cholera toxin. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the species-specific detection and quantitation of V. cholera using a primer pair based on an outer membrane lipoprotein lolB gene for the amplification of a 195 bp DNA fragment. The qPCR primer set for the accurate diagnosis of V. cholera was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.
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