Establishment of Analytical Method for Dichlorprop Residues, a Plant Growth Regulator in Agricultural Commodities Using GC/ECD
2013
Lee, S.M., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Kim, J.Y., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Kim, T.H., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Lee, H.J., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Chang, M.I., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Kim, H.J., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Cho, Y.J., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Choi, S.W., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Kim, M.A., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Kim, M.Y., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Rhee, G.S., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea | Rhee, S.J., Ministry of Food and Drug Safety, Cheongwon , Republic of Korea
BACKGROUND: This study focused on the development of an analytical method about dichlorprop (DCPP; 2-(2,4-dichlorophenoxy)propionic acid) which is a plant growth regulator, a synthetic auxin for agricultural commodities. DCPP prevents falling of fruits during their growth periods. However, the overdose of DCPP caused the unwanted maturing time and reduce the safe storage period. If we take fruits with exceeding maximum residue limits, it could be harmful. Therefore, this study presented the analytical method of DCPP in agricultural commodities for the nation-wide pesticide residues monitoring program of Ministry of Food and Drug Safety. METHODS AND RESULTS: We adopted the analytical method for DCPP in agricultural commodities by gas chromatograph in cooperated with Electron Capture Detector (ECD). Sample extraction and purification by ion-associated partition method were applied, then quantitation was done by GC/ECD with DB-17, a moderate polarity column under the temperature-rising condition with nitrogen as a carrier gas and split-less mode. Standard calibration curve presented linearity with the correlation coefficient (r2) 0.9998, analysed from 0.1 to 2.0 mg/L concentration. Limit of quantitation in agricultural commodities represents represents 0.05 mg/kg, and average recoveries ranged from 78.8 to 102.2%. The repeatability of measurements expressed as coefficient of variation (CV%) was less than 9.5% in 0.05, 0.10, and 0.50 mg/kg. CONCLUSION(S): Our newly improved analytical method for DCPP residues in agricultural commodities was applicable to the nation-wide pesticide residues monitoring program with the acceptable level of sensitivity, repeatability, and reproducibility.
显示更多 [+] 显示较少 [-]