Method of Rearing Queens on Wax Cups of Apis cerana F.
2013
Choi, Y.S., RDA, Suwon, Republic of korea | Lee, M.Y., RDA, Suwon, Republic of korea | Hong, I.P., RDA, Suwon, Republic of korea | Woo, S.O., RDA, Suwon, Republic of korea | Shim, H.S., RDA, Suwon, Republic of korea | Byun, K.H., RDA, Suwon, Republic of korea | Thapa, R., RDA, Suwon, Republic of korea | Lee, M.L., RDA, Suwon, Republic of korea
We compared the grafting success to rear of Apis carana F. queens cells, to which we grafted 540 larvae. The wax for cups we prepared from A. mellifera L. and A. cerana F. wax. The A. cerana F. wax cups were found that artificial queen cell cups with the internal diameter of 8.0 mm at the mouth and 8.0 mm depth were highly preferred by the bees for rearing of queens from the grafted larvae. From the 180 grafted larvae into A. mellifera L. wax bees accepted 30 queens cells, only (16.66%); A. cerana wax bees accepted 59 queens cells (32.77%); plastic cup bees accepted 18 queens cells, only (10.00%). In the preference test the grafting success in the A. cerana wax cups were better than in the A. mellifera L. wax and plastic cup. The results show better acceptance of larvae grafted into the pure A. cerana F. wax cups for rearing A. cerana F. queen. A new method for rearing honey bees, A. cerana F., in vivo was developed and the effects of royal jelly from A. mellifera L. We used royal jelly diluted 50:50 with sterile water (The royal jelly is kept frozen until used). A small amount (±10μg) of royal jelly is placed at the center of each cell cup. Young A. cerana larvae were transferred into the queen cups containing ±10μg of the Royal jelly from A. mellifera L. and A. cerana F. The average rates of acceptance were affected significantly due to the royal jelly source in the queen cell cups (over 90%). It is so workable first to produce pure A. cerana F. wax for making the queen cups before a beekeeper starts with grafting.
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